UBA2 antibody (AA 449-564)

Catalog No. ABIN7601756

This anti-UBA2 antibody is a Mouse Monoclonal antibody detecting UBA2 in WB and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for UBA2 antibody (AA 449-564) (ABIN7601756)

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBA2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 5H11

Product Details anti-UBA2 Antibody

Binding Specificity: AA 449-564

Purpose: Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) (ABIN7601756). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human SAE2/UBA2 recombinant protein (Position: E449-K564).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: ubiquitin-like modifier activating enzyme 2". 2. Gong L, Li B, Millas S, Yeh ET (April 1999). "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex". FEBS Lett. 448 (1): 185-9. 3. Desterro JM, Rodriguez MS, Kemp GD, Hay RT (April 1999). "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1". J. Biol. Chem. 274 (15): 10618-24.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for UBA2

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Alternative Name: UBA2

Background:

Synonyms: SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, UBA2, SAE2, UBLE1B, HRIHFB2115

Background: Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins

Molecular Weight: 90 kDa

Gene ID: 10054