POLE2 antibody (AA 46-483)

Catalog No. ABIN7601802

Product Details anti-POLE2 Antibody

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Binding Specificity: AA 46-483

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-POLE2 Antibody Picoband®

Characteristics: Anti-POLE2 Antibody Picoband® (ABIN7601802). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLE2 recombinant protein (Position: E46-K483). Human POLE2 shares 99.1% amino acid (aa) sequence identity with mouse POLE2.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Frugoni, F., Dobbs, K., Felgentreff, K., Aldhekri, H., Al Saud, B. K., Arnaout, R., Ali, A. A., Abhyankar, A., Alroqi, F., Giliani, S., Ojeda, M. M., Tsitsikov, E., Pai, S.-Y., Casanova, J. L., Notarangelo, L. D., Manis, J. P. A novel mutation in the POLE2 gene causing combined immunodeficiency. J. Allergy Clin. Immun. 137: 635-638, 2016. 2. Li, Y., Asahara, H., Patel, V. S., Zhou, S., Linn, S. Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. J. Biol. Chem. 272: 32337-32344, 1997. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 03/26/2021.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLE2

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Alternative Name: POLE2 (POLE2 Products)

Background: DNA polymerase epsilon subunit 2 is an enzyme that in humans is encoded by the POLE2 gene. DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.

Gene ID: 5427

UniProt: P56282

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA