POLE2 antibody (AA 46-483)

Catalog No. ABIN7601802

This Rabbit Polyclonal antibody specifically detects POLE2 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for POLE2 antibody (AA 46-483) (ABIN7601802)

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-POLE2 Antibody

Binding Specificity: AA 46-483

Purpose: Anti-POLE2 Antibody

Characteristics: Anti-POLE2 Antibody (ABIN7601802). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLE2 recombinant protein (Position: E46-K483). Human POLE2 shares 99.1% amino acid (aa) sequence identity with mouse POLE2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLE2

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Alternative Name: POLE2

Background: DNA polymerase epsilon subunit 2 is an enzyme that in humans is encoded by the POLE2 gene. DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.

Gene ID: 5427

UniProt: P56282

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA