AXL antibody (AA 47-244)

Catalog No. ABIN7601813

This anti-AXL antibody is a Rabbit Polyclonal antibody detecting AXL in WB, ELISA and FACS. Suitable for Human.

Quick Overview for AXL antibody (AA 47-244) (ABIN7601813)

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AXL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-AXL Antibody

Binding Specificity: AA 47-244

Purpose: Anti-AXL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AXL Antibody Picoband® (ABIN7601813). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AXL recombinant protein (Position: A47-A244).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Temperton, N. J., Wright, E. Retroviral pseudotypes. In: Encyclopedia of Life Sciences. Chichester, England: John Wiley & Sons, Ltd. 2009. 2. Varnum, B. C., Young, C., Elliott, G., Garcia, A., Bartley, T. D., Fridell, Y.-W., Hunt, R. W., Trail, G., Clogston, C., Toso, R. J., Yanagihara, D., Bennett, L., Sylber, M., Merewether, L. A., Tseng, A., Escobar, E., Liu, E. T., Yamane, H. K. Axl receptor tyrosine kinase stimulated by the vitamin K-dependent protein encoded by growth-arrest-specific gene 6. Nature 373: 623-626, 1995. 3. Zhang, Z., Lee, J. C., Lin, L., Olivas, V., Au, V., LaFramboise, T., Abdel-Rahman, M., Wang, X., Levine, A. D., Rho, J. K., Choi, Y. J., Choi, C.-M., and 18 others. Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Nature Genet. 44: 852-860, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AXL

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Alternative Name: AXL

Background:

Synonyms: Tyrosine-protein kinase receptor UFO (EC:2.7.10.1), AXL oncogene, AXL, UFO

Tissue Specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Background: Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. This gene is mapped to 19q13.2. The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Molecular Weight: 140 kDa

Gene ID: 558

UniProt: P30530

Pathways: RTK Signaling, Cellular Response to Molecule of Bacterial Origin