Lamin A/C antibody (AA 481-646)

Catalog No. ABIN7601843

This anti-Lamin A/C antibody is a Mouse Monoclonal antibody detecting Lamin A/C in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for Lamin A/C antibody (AA 481-646) (ABIN7601843)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 5F3C12

Product Details anti-Lamin A/C Antibody

Binding Specificity: AA 481-646

Purpose: Anti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) (ABIN7601843). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Lammerding, J., Schulze, P. C., Takahashi, T., Kozlov, S., Sullivan, T., Kamm, R. D., Stewart, C. L., Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004. 2. Lin, F., Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993. 3. Wydner, K. L., McNeil, J. A., Lin, F., Worman, H. J., Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: LMNA

Background:

Synonyms: E3 SUMO protein ligase TRIM28 antibody, E3 SUMO-protein ligase TRIM28 antibody, FLJ29029 antibody, KAP 1 antibody, KAP-1 antibody, KRAB associated protein 1 antibody, KRAB interacting protein 1 antibody, KRAB-associated protein 1 antibody, KRAB-interacting protein 1 antibody, KRIP 1 antibody, KRIP-1 antibody, KRIP1 antibody, Nuclear corepressor KAP 1 antibody, Nuclear corepressor KAP-1 antibody, RING finger protein 96 antibody, RNF96 antibody, TF1B antibody, TIF1 beta antibody, TIF1-beta antibody, TIF1B antibody, TIF1B_HUMAN antibody, Transcription intermediary factor 1 beta antibody, Transcription intermediary factor 1-beta antibody, Trim28 antibody, Tripartite motif containing 28 antibody, tripartite motif containing protein 28 antibody, Tripartite motif-containing protein 28 antibody

Tissue Specificity: Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Background: Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Molecular Weight: 74 kDa

Gene ID: 4000

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus