TXNIP antibody (AA 5-391)

Catalog No. ABIN7601882

This anti-TXNIP antibody is a Rabbit Polyclonal antibody detecting TXNIP in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for TXNIP antibody (AA 5-391) (ABIN7601882)

Target: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TXNIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-TXNIP Antibody

Binding Specificity: AA 5-391

Purpose: Anti-TXNIP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TXNIP Antibody Picoband® (ABIN7601882). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TXNIP recombinant protein (Position: K5-Q391).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bodnar, J. S., Chatterjee, A., Castellani, L. W., Ross, D. A., Ohmen, J., Cavalcoli, J., Wu, C., Dains, K. M., Catanese, J., Chu, M., Sheth, S. S., Charugundla, K., Demant, P., West, D. B., de Jong, P., Lusis, A. J. Positional cloning of the combined hyperlipidemia gene Hyplip1. Nature Genet. 30: 110-116, 2002. 2. Hui, S. T. Y., Andres, A. M., Miller, A. K., Spann, N. J., Potter, D. W., Post, N. M., Chen, A. Z., Sachithanantham, S., Jung, D. Y., Kim, J. K., Davis, R. A. Txnip balances metabolic and growth signaling via PTEN disulfide reduction. Proc. Nat. Acad. Sci. 105: 3921-3926, 2008. 3. Suh, H.-W., Yun, S., Song, H., Jung, H., Park, Y.-J., Kim, T.-D., Yoon, S. R., Choi, I. TXNIP interacts with hEcd to increase p53 stability and activity. Biochem. Biophys. Res. Commun. 438: 264-269, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TXNIP

Target: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Alternative Name: TXNIP

Background:

Synonyms: Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1, TXNIP, VDUP1

Tissue Specificity: Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

Background: Thioredoxin-interacting protein is a protein that in humans is encoded by the TXNIP gene. It is mapped to 1q21.1. This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants.

Molecular Weight: 44 kDa

Gene ID: 10628

UniProt: Q9H3M7

Pathways: Protein targeting to Nucleus, Platelet-derived growth Factor Receptor Signaling, Inflammasome