PIK3 gamma antibody (AA 52-308)

Catalog No. ABIN7601952

This Rabbit Polyclonal antibody specifically detects PIK3 gamma in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIK3 gamma antibody (AA 52-308) (ABIN7601952)

Target: PIK3 gamma (PIK3CG) (Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3 gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PIK3 gamma Antibody

Binding Specificity: AA 52-308

Purpose: Anti-PI3K-gamma/PIK3CG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PI3K-gamma/PIK3CG Antibody Picoband® (ABIN7601952). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PI3K-gamma/PIK3CG recombinant protein (Position: K52-D308).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anzelon, A. N., Wu, H., Rickert, R. C. Pten inactivation alters peripheral B lymphocyte fate and reconstitutes CD19 function. Nature Immun. 4: 287-294, 2003. 2. Barber, D. F., Bartolome, A., Hernandez, C., Flores, J. M., Redondo, C., Fernandez-Arias, C., Camps, M., Ruckle, T., Schwarz, M. K., Rodriguez, S., Martinez-A, C., Balomenos, D., Rommel, C., Carrera, A. C. PI3K-gamma inhibition blocks glomerulonephritis and extends lifespan in a mouse model of systemic lupus. Nature Med. 11: 933-935, 2005. 3. Barbier, M., Attoub, S., Calvez, R., Laffargue, M., Jarry, A., Mareel, M., Altruda, F., Gespach, C., Wu, D., Lu, B., Hirsch, E., Wymann, M. P. Weakening link to colorectal cancer? Nature 413: 796 only, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PIK3 gamma

Target: PIK3 gamma (PIK3CG) (Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG))

Alternative Name: PIK3CG

Background:

Synonyms: Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46, CD335, NCR1, LY94

Tissue Specificity: Selectively expressed by both resting and activated NK cells.

Background: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform is an enzyme that in humans is encoded by the PIK3CG gene. Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 126 kDa

Gene ID: 5294

UniProt: P48736

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Inositol Metabolic Process, Hepatitis C, VEGF Signaling