JUP antibody (AA 556-745)

Catalog No. ABIN7602031

The Mouse Monoclonal anti-JUP antibody has been validated for WB, IF, IHC, FACS and ICC. It is suitable to detect JUP in samples from Human, Mouse and Rat.

Quick Overview for JUP antibody (AA 556-745) (ABIN7602031)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 4C12D7

Product Details anti-JUP Antibody

Binding Specificity: AA 556-745

Purpose: Anti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) (ABIN7602031). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745). Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kolligs, F. T., Kolligs, B., Hajra, K. M., Hu, G., Tani, M., Cho, K. R., Fearon, E. R. : Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin. Genes Dev. 14: 1319-1331, 2000. 2. "Entrez Gene: JUP junction plakoglobin"

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Background:

Synonyms: Ras-related protein Rab-5A, RAB5A, RAB5

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: Junction plakoglobin(JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to β-catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.

Molecular Weight: 82 kDa

Gene ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location