FBXO32 antibody (AA 6-355)

Catalog No. ABIN7602129

This Rabbit Polyclonal antibody specifically detects FBXO32 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for FBXO32 antibody (AA 6-355) (ABIN7602129)

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-FBXO32 Antibody

Binding Specificity: AA 6-355

Purpose: Anti-FBXO32 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-FBXO32 Antibody. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FBXO32 recombinant protein (Position: Q6-F355).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FBXO32

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Alternative Name: FBXO32

Background:

Background: F-box only protein 32, also known as MAFbx, for Muscle Atrophy F-box gene, and Atrogin-1, is a protein that in humans is encoded by the FBXO32 gene. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: F-box protein 32

Molecular Weight: 42 kDa

Gene ID: 114907

UniProt: Q969P5