DUOX2 antibody (AA 63-403)

Catalog No. ABIN7602208

Product Details anti-DUOX2 Antibody

Target: DUOX2 (Dual Oxidase 2 (DUOX2))

Binding Specificity: AA 63-403

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Anti-Duox2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Duox2 Antibody Picoband® (ABIN7602208). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Duox2 recombinant protein (Position: A63-D403).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Caillou, B., Dupuy, C., Lacroix, L., Nocera, M., Talbot, M., Ohayon, R., Deme, D., Bidart, J.-M., Schlumberger, M., Virion, A. Expression of reduced nicotinamide adenine dinucleotide phosphate oxidase (ThoX, LNOX, Duox) genes and proteins in human thyroid tissues. J. Clin. Endocr. Metab. 86: 3351-3358, 2001. 2. De Deken, X., Wang, D., Many, M.-C., Costagliola, S., Libert, F., Vassart, G., Dumont, J. E., Miot, F. Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family. J. Biol. Chem. 275: 23227-23233, 2000. 3. Dupuy, C., Ohayon, R., Valent, A., Noel-Hudson, M.-S., Deme, D., Virion, A. Purification of a novel flavoprotein involved in the thyroid NADPH oxidase: cloning of the porcine and human cDNAs. J. Biol. Chem. 274: 37265-37269, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DUOX2

Target: DUOX2 (Dual Oxidase 2 (DUOX2))

Alternative Name: Duox2 (DUOX2 Products)

Background:

Synonyms: Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235a, GYPA, GPA

Tissue Specificity: Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Background: Dual oxidase 2, also known as DUOX2 or ThOX2 (for thyroid oxidase), is an enzyme that in humans is encoded by the DUOX2 gene. The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX1. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.

Molecular Weight: 175 kDa

Gene ID: 214593

Pathways: Thyroid Hormone Synthesis