SGOL2 antibody (AA 660-1011)

Catalog No. ABIN7602258

This Rabbit Polyclonal antibody specifically detects SGOL2 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for SGOL2 antibody (AA 660-1011) (ABIN7602258)

Target: SGOL2 (Shugoshin-Like 2 (SGOL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGOL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SGOL2 Antibody

Binding Specificity: AA 660-1011

Purpose: Anti-SGO2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SGO2 Antibody Picoband® (ABIN7602258). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SGO2 recombinant protein (Position: N660-E1011).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 11/20/2008. 2. Hellmuth, S., Gomez-H, L., Pendas, A. M., Stemmann, O. Securin-independent regulation of separase by checkpoint-induced shugoshin-MAD2. Nature 580: 536-541, 2020. 3. Huang, H., Feng, J., Famulski, J., Rattner, J. B., Liu, S. T., Kao, G. D., Muschel, R., Chan, G. K. T., Yen, T. J. Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments. J. Cell Biol. 177: 413-424, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SGOL2

Target: SGOL2 (Shugoshin-Like 2 (SGOL2))

Alternative Name: SGO2

Background:

Synonyms: Gap junction gamma-1 protein,Connexin-45,Cx45,Gap junction alpha-7 protein,GJC1,GJA7,

Tissue Specificity: Ubiquitously expressed.

Background: Predicted to be involved in homologous chromosome segregation, meiotic sister chromatid cohesion, and mitotic sister chromatid segregation. Predicted to act upstream of or within meiotic nuclear division, positive regulation of maintenance of meiotic sister chromatid cohesion, centromeric, and protein localization. Located in chromosome, centromeric region and nuclear body. Part of mitotic cohesin complex.

Molecular Weight: 180 kDa

Gene ID: 151246

UniProt: Q562F6