Calnexin antibody (AA 68-582)

Catalog No. ABIN7602289

The Mouse Monoclonal anti-Calnexin antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect Calnexin in samples from Human, Mouse and Rat.

Quick Overview for Calnexin antibody (AA 68-582) (ABIN7602289)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 8D10B3

Product Details anti-Calnexin Antibody

Binding Specificity: AA 68-582

Purpose: Anti-Calnexin/CANX Antibody (monoclonal, 8D10B3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Calnexin/CANX Antibody (monoclonal, 8D10B3) (ABIN7602289). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calnexin/CANX recombinant protein (Position: E68-R582).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX

Background:

Synonyms: Cadherin-3, Placental cadherin, P-cadherin, CDH3, CDHP

Tissue Specificity: Expressed in some normal epithelial tissues and in some carcinoma cell lines.

Background: Calnexin (CNX) is a 67 kDa integral protein of the endoplasmic reticulum. This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 95 kDa

Gene ID: 821

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis