Calnexin antibody (AA 68-582)

Catalog No. ABIN7602290

This anti-Calnexin antibody is a Rabbit Polyclonal antibody detecting Calnexin in WB, IHC, IF, ELISA, FACS and ICC. Suitable for Human and Mouse.

Quick Overview for Calnexin antibody (AA 68-582) (ABIN7602290)

Target: Calnexin (CANX)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Calnexin Antibody

Binding Specificity: AA 68-582

Purpose: Anti-Calnexin/CANX Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Calnexin/CANX Antibody Picoband® (ABIN7602290). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calnexin/CANX recombinant protein (Position: E68-R582).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Denzel, A., Molinari, M., Trigueros, C., Martin, J. E., Velmurgan, S., Brown, S., Stamp, G., Owen, M. J. Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression. Molec. Cell. Biol. 22: 7398-7404, 2002. 2. Dickson, K. M., Bergeron, J. J. M., Shames, I., Colby, J., Nguyen, D. T., Chevet, E., Thomas, D. Y., Snipes, G. J. Association of calnexin with mutant peripheral myelin protein-22 ex vivo: a basis for 'gain-of-function' ER diseases. Proc. Nat. Acad. Sci. 99: 9852-9857, 2002. 3. Gray, P. W., Byers, M. G., Eddy, R. L., Shows, T. B. The assignment of the calnexin gene to the q35 region of chromosome 5. (Abstract) Human Genome Mapping Workshop 93, Kobe, Japan 1993. P. 9.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX

Background:

Synonyms: Vitamin D-binding protein, DBP, VDB, Gc-globulin, Group-specific component, Gc

Background: Calnexin (CNX) is a 67 kDa integral protein of the endoplasmic reticulum. This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 97 kDa

Gene ID: 821

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis