BARD1 antibody (AA 709-758)

Catalog No. ABIN7602360

This Rabbit Polyclonal antibody specifically detects BARD1 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BARD1 antibody (AA 709-758) (ABIN7602360)

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BARD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-BARD1 Antibody

Binding Specificity: AA 709-758

Purpose: Anti-BARD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BARD1 Antibody Picoband® (ABIN7602360). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BARD1 recombinant protein (Position: P709-A758).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Daza-Martin, M., Starowicz, K., Jamshad, M., Tye, S., Ronson, G. E., MacKay, H. L., Chauhan, A. S., Walker, A.K., Stone, H. R., Beesley, J. F. J., Coles, J. L., Garvin, A. J., Stewart, G. S., McCorvie, T. J., Zhang, X., Densham, R. M., Morris, J. R. Isomerization of BRCA1-BARD1 promotes replication fork protection. Nature 571: 521-527, 2019. 2. Dong, Y., Hakimi, M.-A., Chen, X., Kumaraswamy, E., Cooch, N. S., Godwin, A. K., Shiekhattar, R. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair. Molec. Cell 12: 1087-1099, 2003. 3. Irminger-Finger, I., Leung, W.-C., Li, J., Dubois-Dauphin, M., Harb, J., Feki, A., Jefford, C. E., Soriano, J. V., Jaconi, M., Montesano, R., Krause, K.-H. Identification of BARD1 as mediator between proapoptotic stress and p53-dependent apoptosis. Molec. Cell 8: 1255-1266, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BARD1

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Alternative Name: BARD1

Background:

Synonyms: RNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2

Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

Background: BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 87 kDa

Gene ID: 580

UniProt: Q99728

Pathways: DNA Damage Repair