SIN3A antibody (AA 8-918)

Catalog No. ABIN7602504

This Rabbit Polyclonal antibody specifically detects SIN3A in WB, ELISA, IF, IHC, ICC and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for SIN3A antibody (AA 8-918) (ABIN7602504)

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SIN3A Antibody

Binding Specificity: AA 8-918

Purpose: Anti-mSin3A/SIN3A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-mSin3A/SIN3A Antibody Picoband® (ABIN7602504). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human mSin3A/SIN3A recombinant protein (Position: Q8-E918).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ayer, D. E., Lawrence, Q. A., Eisenman, R. N. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3. Cell 80: 767-776, 1995. 2. Balasubramanian, M., Dingemans, A. J. M., Albaba, S., Richardson, R., Yates, T. M., Cox, H., Douzgou, S., Armstrong, R., Sansbury, F. H., Burke, K. B., Fry, A. E., Ragge, N., and 27 others. Comprehensive study of 28 individuals with SIN3A-related disorder underscoring the associated mild cognitive and distinctive facial phenotype. Europ. J. Hum. Genet. 29: 625-636, 2021. 3. Duong, H. A., Robles, M. S., Knutti, D., Weitz, C. J. A molecular mechanism for circadian clock negative feedback. Science 332: 1436-1439, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SIN3A

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Alternative Name: SIN3A

Background:

Synonyms: Microtubule-associated protein 2, MAP-2, MAP2

Background: Paired amphipathic helix protein Sin3a is a protein that in humans is encoded by the SIN3A gene. The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Molecular Weight: 150 kDa

Gene ID: 25942

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha