NYS48/HAUS8 antibody (AA 86-322)

Catalog No. ABIN7602570

This Rabbit Polyclonal antibody specifically detects NYS48/HAUS8 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for NYS48/HAUS8 antibody (AA 86-322) (ABIN7602570)

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NYS48/HAUS8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NYS48/HAUS8 Antibody

Binding Specificity: AA 86-322

Purpose: Anti-HAUS8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HAUS8 Antibody Picoband® (ABIN7602570). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HAUS8 recombinant protein (Position: D86-A322). Human HAUS8 shares r54.1% amino acid (aa) sequence identity with mouse HAUS8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Goshima, G., Mayer, M., Zhang, N., Stuurman, N., Vale, R. D. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J. Cell Biol. 181: 421-429, 2008. 2. Lawo, S., Bashkurov, M., Mullin, M., Gomez Ferreria, M., Kittler, R., Habermann, B., Tagliaferro, A., Poser, I., Hutchins, J. R. A., Hegemann, B., Pinchev, D., Buchholz, F., Peters, J.-M., Hyman, A. A., Gingras, A.-C., Pelletier, L. HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity. Curr. Biol. 19: 816-826, 2009. 3. Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R. D., Obuse, C., Goshima, G. The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc. Nat. Acad. Sci. 106: 6998-7003, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NYS48/HAUS8

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Alternative Name: HAUS8

Background:

Synonyms: HAUS8, HICE1, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48

Background: HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly.

Molecular Weight: 45 kDa

Gene ID: 93323