PSPC1 antibody (AA 96-489)

Catalog No. ABIN7602700

The Rabbit Polyclonal anti-PSPC1 antibody has been validated for WB, ELISA, IHC, ICC, IF and FACS. It is suitable to detect PSPC1 in samples from Human, Mouse and Rat.

Quick Overview for PSPC1 antibody (AA 96-489) (ABIN7602700)

Target: PSPC1 (Paraspeckle Component 1 (PSPC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSPC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PSPC1 Antibody

Binding Specificity: AA 96-489

Purpose: Anti-PSPC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PSPC1 Antibody Picoband® (ABIN7602700). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PSPC1 recombinant protein (Position: E96-Q489).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Andersen, J. S., Lyon, C. E., Fox, A. H., Leung, A. K. L., Lam, Y. W., Steen, H., Mann, M., Lamond, A. I. ed proteomic analysis of the human nucleolus. Curr. Biol. 12: 1-11, 2002. 2. Fox, A. H., Bond, C. S., Lamond, A. I. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Molec. Biol. Cell 16: 5304-5315, 2005. 3. Fox, A. H., Lam, Y. W., Leung, A. K. L., Lyon, C. E., Andersen, J., Mann, M., Lamond, A. I. Paraspeckles: a novel nuclear domain. Curr. Biol. 12: 13-25, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PSPC1

Target: PSPC1 (Paraspeckle Component 1 (PSPC1))

Alternative Name: PSPC1

Background:

Synonyms: Rho-related GTP-binding protein Rho6,Rho family GTPase 1,Rnd1,RND1,RHO6,

Tissue Specificity: Mostly expressed in brain and liver.

Background: This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified.

Molecular Weight: 66 kDa

Gene ID: 55269