APOA5 antibody (AA 99-217)

Catalog No. ABIN7602720

This Rabbit Polyclonal antibody specifically detects APOA5 in WB, ELISA and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for APOA5 antibody (AA 99-217) (ABIN7602720)

Target: APOA5 (Apolipoprotein A-V (APOA5))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-APOA5 Antibody

Binding Specificity: AA 99-217

Purpose: Anti-Apolipoprotein A V/APOA5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Apolipoprotein A V/APOA5 Antibody Picoband® (ABIN7602720). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Apolipoprotein A V/APOA5 recombinant protein (Position: E99-A217).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1.Caussy, C., Charriere, S., Marcais, C., Di Filippo, M., Sassolas, A., Delay, M., Euthine, V., Jalabert, A., Lefai, E., Rome, S., Moulin, P. An APOA5 3-prime UTR variant associated with plasma triglycerides triggers APOA5 downregulation by creating a functional miR-485-5p binding site. Am. J. Hum. Genet. 94: 129-134, 2014. 2.Do, R., Stitziel, N. O., Won, H.-H., Berg Jorgensen, A., Duga, S., Merlini, P. A., Kiezun, A., Farrall, M., Goel, A., Zuk, O., Guella, I., Asselta, R., and 82 others. Exome sequencing identifies rare LDLR and APOA5 alleles conferring risk for myocardial infarction. Nature 518: 102-106, 2015. 3.Endo, K., Yanagi, H., Araki, J., Hirano, C., Yamakawa-Kobayashi, K., Tomura, S. Association found between the promoter region polymorphism in the apolipoprotein A-V gene and the serum triglyceride level in Japanese schoolchildren. Hum. Genet. 111: 570-572, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for APOA5

Target: APOA5 (Apolipoprotein A-V (APOA5))

Alternative Name: APOA5

Background:

Synonyms: P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P, Selp, Grmp

Tissue Specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Background: Apolipoprotein A-V is a protein that in humans is encoded by the APOA5 gene. The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified.

Molecular Weight: 45 kDa

Gene ID: 116519

UniProt: Q6Q788

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism