TUBB antibody (C-Term)

Catalog No. ABIN7602744

This Mouse Monoclonal antibody specifically detects TUBB in WB, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TUBB antibody (C-Term) (ABIN7602744)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2E11

Product Details anti-TUBB Antibody

Binding Specificity: C-Term

Purpose: Anti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) (ABIN7602744). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence,2 μg/mL,Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: TUBB tubulin, beta". 2. Volz A, Weiss E, Trowsdale J, Ziegler A (Jan 1994). "Presence of an expressed beta-tubulin gene (TUBB) in the HLA class I region may provide the genetic basis for HLA-linked microtubule dysfunction". Human Genetics. 93 (1): 42-6.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB

Background:

Synonyms: Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5, OK/SW-cl.56

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Molecular Weight: 55 kDa

Gene ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase