NOD1 antibody (C-Term)

Catalog No. ABIN7602813

This Rabbit Polyclonal antibody specifically detects NOD1 in WB. It exhibits reactivity toward Monkey.

Quick Overview for NOD1 antibody (C-Term) (ABIN7602813)

Target: NOD1 (Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1))

Reactivity: Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOD1 Antibody

Binding Specificity: C-Term

Purpose: Anti-CARD4/NOD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CARD4/NOD1 Antibody Picoband® (ABIN7602813). Tested in WB applications. This antibody reacts with Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CARD4/NOD1, which shares 88.2% and 93.8% amino acid (aa) sequence identity with mouse and rat CARD4/NOD1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Monkey
1. Chamaillard, M., Hashimoto, M., Horie, Y., Masumoto, J., Su, Q., Saab, L., Ogura, Y., Kawasaki, A., Fukase, K., Kusumoto, S., Valvano, M. A., Foster, S. J., Mak, T. W., Nunez, G., Inohara, N. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nature Immun. 4: 702-707, 2003. 2. da Silva Correia, J., Miranda, Y., Austin-Brown, N., Hsu, J., Mathison, J., Xiang, R., Zhou, H., Li, Q., Han, J., Ulevitch, R. J. Nod1-dependent control of tumor growth. Proc. Nat. Acad. Sci. 103: 1840-1845, 2006. 3. Martinon F, Tschopp J (2006). "NLRs join TLRs as innate sensors of pathogens.".Trends Immunol. 26 (8): 447-54.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NOD1

Target: NOD1 (Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1))

Alternative Name: NOD1

Background:

Synonyms: Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk, SYK

Tissue Specificity: Widely expressed in hematopoietic cells. Expressed in neutrophils. Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.

Background: Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is mapped to 7p14.3. It recognizes bacterial molecules and stimulates an immune reaction. NOD1 protein contains a caspase recruitment domain (CARD). This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. What wore, it has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.

Molecular Weight: 108 kDa

Gene ID: 10392

UniProt: Q9Y239

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Inflammasome