PNP antibody (Middle Region)

Catalog No. ABIN7602996

This Mouse Monoclonal antibody specifically detects PNP in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for PNP antibody (Middle Region) (ABIN7602996)

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PNP antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2H10

Product Details anti-PNP Antibody

Binding Specificity: Middle Region

Purpose: Anti-PNP Antibody (monoclonal, 2H10)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by six amino acids,and from the related rat sequence by five amino acids.

Characteristics: Anti-PNP Antibody.(monoclonal, 2H10)-1. Tested in WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PNP, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PNP

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Alternative Name: PNP

Background:

Background: The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised Tlymphocyte function and neurologic dysfunction.

Gene Full Name: purine nucleoside phosphorylase

Molecular Weight: 32 kDa

Gene ID: 4860

UniProt: P00491

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus