SUMO1 antibody (Middle Region)

Catalog No. ABIN7603002

This Rabbit Polyclonal antibody specifically detects SUMO1 in IF, IHC, FACS and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SUMO1 antibody (Middle Region) (ABIN7603002)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUMO1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SUMO1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Sumo 1/SUMO1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Sumo 1/SUMO1 Antibody Picoband® (ABIN7603002). Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Sumo 1/SUMO1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
1. Alkuraya, F. S., Saadi, I., Lund, J. L., Turbe-Doan, A., Morton, C. C., Maas, R. L. SUMO1 haploinsufficiency leads to cleft lip and palate. Science 313: 1751 only, 2006. 2. Kho, C., Lee, A., Jeong, D., Oh, J. G., Chaanine, A. H., Kizana, E., Park, W. J., Hajjar, R. J. SUMO1-dependent modulation of SERCA2a in heart failure. Nature 477: 601-605, 2011. 3. Martin, S., Nishimune, A., Mellor, J. R., Henley, J. M. SUMOylation regulates kainate-receptor-mediated synaptic transmission. Nature 447: 321-325, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1

Background:

Synonyms: Polycystin-2, PC2, Autosomal dominant polycystic kidney disease type II protein, Polycystic kidney disease 2 protein, Polycystwin, R48321, Transient receptor potential cation channel subfamily P member 2, PKD2, TRPP2

Tissue Specificity: Detected in fetal and adult kidney (PubMed:10770959). Detected at the thick ascending limb of the loop of Henle, at distal tubules, including the distal convoluted tubule and cortical collecting tubules, with weak staining of the collecting duct (PubMed:10770959). Detected on placenta syncytiotrophoblasts (at protein level) (PubMed:26269590). Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes.

Background: Small ubiquitin-related modifier 1 (SUMO1), also called SMT3C or PIC1 is a protein that in humans is encoded by the SUMO1 gene. This gene is mapped to 2q33.1. This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene.

Molecular Weight: 12 kDa

Gene ID: 7341

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway