KCNJ1 antibody (N-Term)

Catalog No. ABIN7603179

This Rabbit Polyclonal antibody specifically detects KCNJ1 in WB. It exhibits reactivity toward Rat, Mouse and Monkey.

Quick Overview for KCNJ1 antibody (N-Term) (ABIN7603179)

Target: KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))

Reactivity: Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNJ1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KCNJ1 Antibody

Binding Specificity: N-Term

Purpose: Anti-KCNJ1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KCNJ1 Antibody Picoband® (ABIN7603179). Tested in WB applications. This antibody reacts with Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KCNJ1, which shares 80% amino acid (aa) sequence identity with both mouse and rat KCNJ1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Monkey, Mouse, Rat
1. Derst, C., Konrad, M., Kockerling, A., Karolyi, L., Deschenes, G., Daut, J., Karschin, A., Seyberth, H. W. Mutations in the ROMK gene in antenatal Bartter syndrome are associated with impaired K(+) channel function. Biochem. Biophys. Res. Commun. 230: 641-645, 1997. 2. Derst, C., Wischmeyer, E., Preisig-Muller, R., Spauschus, A., Konrad, M., Hensen, P., Jeck, N., Seyberth, H. W., Daut, J., Karschin, A. A hyperprostaglandin E syndrome mutation in Kir1.1 (renal outer medullary potassium) channels reveals a crucial residue for channel function in Kir1.3 channels. J. Biol. Chem. 273: 23884-23891, 1998. 3. He, G., Wang, H.-R., Huang, S.-K., Huang, C.-L. Intersectin links WNK kinases to endocytosis of ROMK1. J. Clin. Invest. 117: 1078-1087, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KCNJ1

Target: KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))

Alternative Name: KCNJ1

Background:

Synonyms: Protein S100-A10, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11, S100A10, ANX2LG, CAL1L, CLP11

Background: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 3758

UniProt: P48048