JNK antibody (pThr183, pThr183, pThr221)

Catalog No. ABIN7603384

The Rabbit Monoclonal anti-JNK antibody has been validated for WB, IHC, IF, IP, FACS and ICC. It is suitable to detect JNK in samples from Human, Mouse and Rat.

Quick Overview for JNK antibody (pThr183, pThr183, pThr221) (ABIN7603384)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: EAG-13

Product Details anti-JNK Antibody

Binding Specificity: pThr183, pThr183, pThr221

Purpose: Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody

Characteristics: Anti-Phospho-JNK1/2/3 (++) MAPK8 Monoclonal Antibody (ABIN7603384). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221)

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8

Background:

Synonyms: Red fluorescent protein eqFP611,GFP-like chromoprotein,

Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Molecular Weight: 15 kDa

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins