Cyclin E1 antibody (pThr77)

Catalog No. ABIN7603416

This Rabbit Monoclonal antibody specifically detects Cyclin E1 in WB, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Cyclin E1 antibody (pThr77) (ABIN7603416)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GHC-3

Product Details anti-Cyclin E1 Antibody

Binding Specificity: pThr77

Purpose: Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody

Characteristics: Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody (ABIN7603416). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Cyclin E1

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1

Background:

Synonyms: G1/S-specific cyclin-E1,CCNE1,CCNE,

Tissue Specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. .

Molecular Weight: 185 kDa

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases