Actin antibody

Catalog No. ABIN7603888

The Rabbit Monoclonal anti-Actin antibody has been validated for WB, IHC, IF, FACS, IP and ICC. It is suitable to detect Actin in samples from Human, Mouse and Rat.

Quick Overview for Actin antibody (ABIN7603888)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BE-1

Product Details anti-Actin Antibody

Purpose: Anti-Actin ACTA1 Rabbit Monoclonal Antibody

Characteristics: Anti-Actin ACTA1 Rabbit Monoclonal Antibody (ABIN7603888). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Actin

Isotype: IgG

Application Details

Application Notes: WB 1:3000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background:

Synonyms: Actin, alpha skeletal muscle,Alpha-actin-1,ACTA1,ACTA,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Molecular Weight: 66 kDa

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development