POLB antibody

Catalog No. ABIN7604659

The Rabbit Monoclonal anti-POLB antibody has been validated for WB, IHC, IF, IP and ICC. It is suitable to detect POLB in samples from Human, Mouse and Rat.

Quick Overview for POLB antibody (ABIN7604659)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: ACGH-16

Product Details anti-POLB Antibody

Purpose: Anti-DNA Polymerase beta Monoclonal Antibody

Characteristics: Anti-DNA Polymerase beta Monoclonal Antibody (ABIN7604659). Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB

Background:

Synonyms: Calpain-1 catalytic subunit,3.4.22.52,Calcium-activated neutral proteinase 1,CANP 1,Calpain mu-type,Calpain-1 large subunit,Cell proliferation-inducing gene 30 protein,Micromolar-calpain,muCANP,CAPN1,CANPL1,PIG30,

Tissue Specificity: Ubiquitous.

Molecular Weight: 72 kDa

UniProt: P06746

Pathways: DNA Damage Repair