E2F1 antibody

Catalog No. ABIN7604697

This Rabbit Monoclonal antibody specifically detects E2F1 in WB, IHC, FACS, IP, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for E2F1 antibody (ABIN7604697)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: IGD-5

Product Details anti-E2F1 Antibody

Purpose: Anti-E2F1 Rabbit Monoclonal Antibody

Characteristics: Anti-E2F1 Rabbit Monoclonal Antibody (ABIN7604697). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human E2F1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Synonyms: Transcription factor E2F1,E2F-1,PBR3,Retinoblastoma-associated protein 1,RBAP-1,Retinoblastoma-binding protein 3,RBBP-3,pRB-binding protein E2F-1,E2F1,RBBP3,

Tissue Specificity: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Molecular Weight: 34 kDa

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy