LEF1 antibody

Catalog No. ABIN7605418

The Rabbit Monoclonal anti-LEF1 antibody has been validated for WB, IHC, IF, IP, ICC and FACS. It is suitable to detect LEF1 in samples from Human, Mouse and Rat.

Quick Overview for LEF1 antibody (ABIN7605418)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: CFG-12

Product Details anti-LEF1 Antibody

Purpose: Anti-LEF1 Rabbit Monoclonal Antibody

Characteristics: Anti-LEF1 Rabbit Monoclonal Antibody (ABIN7605418). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human LEF1

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background:

Synonyms: Lymphoid enhancer-binding factor 1,LEF-1,T cell-specific transcription factor 1-alpha,TCF1-alpha,LEF1,

Tissue Specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. .

Molecular Weight: 32-42 kDa, 65 kDa, 75 kDa

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding