MIF antibody

Catalog No. ABIN7605560

The Rabbit Monoclonal anti-MIF antibody (Clone ABAG-13) (ABIN7605560) specifically detects MIF in WB, IP and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MIF antibody (ABIN7605560)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: ABAG-13

Product Details anti-MIF Antibody

Purpose: Anti-MIF Rabbit Monoclonal Antibody

Characteristics: Anti-MIF Rabbit Monoclonal Antibody (ABIN7605560). Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human MIF

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IP 1:20
FC 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background:

Synonyms: Macrophage migration inhibitory factor,MIF,5.3.2.1,Glycosylation-inhibiting factor,GIF,L-dopachrome isomerase,L-dopachrome tautomerase,5.3.3.12,Phenylpyruvate tautomerase,MIF,GLIF, MMIF,

Tissue Specificity: Widely expressed in adult and fetal tissues, very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361, highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract, not detectable in brain.

Molecular Weight: 12 kDa

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling