Cyclin C antibody (AA 23-100) (HRP)

Catalog No. ABIN727259

The Rabbit Polyclonal anti-Cyclin C antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Cyclin C in samples from Human.

Quick Overview for Cyclin C antibody (AA 23-100) (HRP) (ABIN727259)

Target: Cyclin C (CCNC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin C antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin C Antibody (HRP)

Binding Specificity: AA 23-100

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cyclin C

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin C

Target: Cyclin C (CCNC)

Alternative Name: Cyclin C

Background:

Synonyms: CycC, Cyclin-C, SRB11 homolog, hSRB11, CCNC

Background: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.

Gene ID: 892

UniProt: P24863

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha