UBA2 antibody

Catalog No. ABIN7606235

The Rabbit Monoclonal anti-UBA2 antibody (Clone AEAI-21) (ABIN7606235) specifically detects UBA2 in WB, IHC, IF, ICC and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBA2 antibody (ABIN7606235)

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This UBA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: AEAI-21

Product Details anti-UBA2 Antibody

Purpose: Anti-SAE2 / UBA2 Monoclonal Antibody

Characteristics: Anti-SAE2 / UBA2 Monoclonal Antibody (ABIN7606235). Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for UBA2

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Alternative Name: UBA2

Background:

Synonyms: Protein disulfide-isomerase A6,5.3.4.1,Endoplasmic reticulum protein 5,ER protein 5,ERp5,Protein disulfide isomerase P5,Thioredoxin domain-containing protein 7,PDIA6,ERP5, P5, TXNDC7,

Tissue Specificity: Expressed in platelets (at protein level). .

Molecular Weight: 43 kDa