SPHK1 antibody

Catalog No. ABIN7606363

The Rabbit Monoclonal anti-SPHK1 antibody has been validated for WB and FACS. It is suitable to detect SPHK1 in samples from Human, Mouse and Rat.

Quick Overview for SPHK1 antibody (ABIN7606363)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ACCB-19

Product Details anti-SPHK1 Antibody

Purpose: Anti-SPHK1 Monoclonal Antibody

Characteristics: Anti-SPHK1 Monoclonal Antibody (ABIN7606363). Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SPHK1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background:

Synonyms: Interferon-induced, double-stranded RNA-activated protein kinase,2.7.11.1,Eukaryotic translation initiation factor 2-alpha kinase 2,eIF-2A protein kinase 2,Interferon-inducible RNA-dependent protein kinase,P1/eIF-2A protein kinase,Protein kinase RNA-activated,PKR,Protein kinase R ,Tyrosine-protein kinase EIF2AK2,2.7.10.2,p68 kinase,EIF2AK2,PKR, PRKR,

Tissue Specificity: Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression. .

Molecular Weight: 120-140 kDa

Pathways: VEGF Signaling