His Tag antibody

Catalog No. ABIN727295

The Rabbit Polyclonal anti-His Tag antibody has been validated for WB and IHC (p). It is suitable to detect His Tag in samples from Tag, Mouse, Rat and Bacteria. There are 2+ publications available.

Quick Overview for His Tag antibody (ABIN727295)

Target: His Tag

Reactivity: Tag, Mouse, Rat, Bacteria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Cross-Reactivity: Bacteria, Mouse, Rat, Tag

Cross-Reactivity (Details): HHHHHH tag

Characteristics: Antibody is specific for: HHHHHH tag

Purification: Purified by Protein A.

Immunogen: synthetic peptide: HHHHHH conjugated to KLH

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-His Tag antibody (ABIN727295)

Wang, Kan, Yu, Li, Zhang, Pan, Feng: "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." in: Cellular immunology, Vol. 282, Issue 2, pp. 129-35, (2013) (PubMed).

Ma, Lv, Ye, Zhang, Li, Kan, Fan, Liu, Luo, Wang, Yang, Yang, Bai, Lu, Ma, Xi, Wang: "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." in: Molecular pharmaceutics, Vol. 10, Issue 1, pp. 115-26, (2013) (PubMed).

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background:

Synonyms: HisX6, 6His, His Tag, 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Histidine.

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.