His Tag antibody (Biotin)

Catalog No. ABIN7851333

This Rabbit Monoclonal antibody specifically detects His Tag in WB and ELISA.

Quick Overview for His Tag antibody (Biotin) (ABIN7851333)

Target: His Tag

Host: Rabbit

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: DM1

Product Details anti-His Tag Antibody (Biotin)

Purpose: Biotinylated Anti-His Tag Antibody(DM1), Rabbit mAb

Purification: Purified from cell culture supernatant by affinity chromatography

Isotype: IgG

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1), Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteinsin bacteria, insect cells, and mammalian cells.