CD25 antibody (Biotin)

Catalog No. ABIN7608288

The Biotin-conjugated Rabbit Monoclonal anti-CD25 antibody (Clone DM148) (ABIN7608288) specifically detects CD25 in FACS and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD25 antibody (Biotin) (ABIN7608288)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: DM148

Product Details anti-CD25 Antibody (Biotin)

Purpose: Biotinylated Anti-IL2RA antibody(DM148), Rabbit mAb

Purification: Purified from cell culture supernatant by affinity chromatography

Isotype: IgG

Application Details

Application Notes: ELISA 1:5000-10000, Flow Cyt 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA

Background: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation