HMGB1 antibody (Biotin)

Catalog No. ABIN7697204

The Rabbit Polyclonal anti-HMGB1 antibody is suitable to detect HMGB1 in samples from Mouse. It has been validated for WB, IHC and ICC.

Quick Overview for HMGB1 antibody (Biotin) (ABIN7697204)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HMGB1 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)

Specificity: The antibody is a rabbit polyclonal antibody raised against HMGB1. It has been selected for its ability to recognize HMGB1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High Mobility Group Protein 1

Background: HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein

UniProt: P63158

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome