VEGFA antibody (Biotin)

Catalog No. ABIN7702380

The Biotin-conjugated Mouse Monoclonal anti-VEGFA antibody (Clone A8) (ABIN7702380) specifically detects VEGFA in WB, IHC and ICC. The antibody is reactive with Human samples.

Quick Overview for VEGFA antibody (Biotin) (ABIN7702380)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: A8

Product Details anti-VEGFA Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)

Specificity: The antibody is a mouse monoclonal antibody raised against VEGFA. It has been selected for its ability to recognize VEGFA in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: MAA143Hu26Monoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: Vascular Endothelial Growth Factor A

Background: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling