Cyclin D1 antibody

Catalog No. ABIN7637584

This Rabbit Polyclonal antibody specifically detects Cyclin D1 in WB, IHC, IP and ICC. It exhibits reactivity toward Cow.

Quick Overview for Cyclin D1 antibody (ABIN7637584)

Target: Cyclin D1 (CCND1)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Cyclin D1 Antibody

Purpose: Polyclonal Antibody to Cyclin D1 (CCND1)

Specificity: The antibody is a rabbit polyclonal antibody raised against CCND1. It has been selected for its ability to recognize CCND1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background: CCN-D1, BCL1, PRAD1, U21B31, G1/S-Specific Cyclin-D1, Parathyroid Adenomatosis 1, B-Cell CLL/Lymphoma 1, G1/S-Specific Cyclin D1, PRAD1 oncogene

UniProt: Q2KI22

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling