PERK antibody

Catalog No. ABIN7638520

This Rabbit Polyclonal antibody specifically detects PERK in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for PERK antibody (ABIN7638520)

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PERK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PERK Antibody

Purpose: Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Specificity: The antibody is a rabbit polyclonal antibody raised against EIF2aK3. It has been selected for its ability to recognize EIF2aK3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPE975Mu02Recombinant Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PERK

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Alternative Name: EIF2aK3

Background: WRS, PEK, PERK, HsPEK, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase

UniProt: Q9Z2B5

Pathways: Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response