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His Tag antibody

This Rabbit Polyclonal antibody specifically detects His Tag in WB, IP, IHC and ICC. It exhibits reactivity toward Various Species.
Catalog No. ABIN7640269

Quick Overview for His Tag antibody (ABIN7640269)

Target

See all His Tag Antibodies
His Tag

Reactivity

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  • 4
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Various Species

Host

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  • 2
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Rabbit

Clonality

  • 122
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Polyclonal

Conjugate

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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
  • 1
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  • 1
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  • 1
  • 1
  • 1
  • 1
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  • 1
  • 1
  • 1
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  • 1
This His Tag antibody is un-conjugated

Application

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Purpose

    Polyclonal Antibody to Hexa Histidine (H6)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against H6. It has been selected for its ability to recognize H6 in ELISA and CLIA.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    CPX656Ge21OVA Conjugated Hexa Histidine (H6)

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    His Tag

    Alternative Name

    Hexa Histidine

    Target Type

    Tag

    Background

    6xHis, His-Tag
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