Insulin antibody

Catalog No. ABIN7640594

The Mouse Monoclonal anti-Insulin antibody has been validated for IHC, WB, IP and ICC. It is suitable to detect Insulin in samples from Human.

Quick Overview for Insulin antibody (ABIN7640594)

Target: Insulin (INS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: H10

Product Details anti-Insulin Antibody

Purpose: Monoclonal Antibody to Insulin (INS)

Specificity: The antibody is a mouse monoclonal antibody raised against INS. It has been selected for its ability to recognize INS in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPA448Hu01Recombinant Insulin (INS)

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation