CD11b antibody (AA 77-180)

Catalog No. ABIN730918

This Rabbit Polyclonal antibody specifically detects CD11b in FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 10+ publications.

Quick Overview for CD11b antibody (AA 77-180) (ABIN730918)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD11b Antibody

Binding Specificity: AA 77-180

Specificity: The immunogen of this product has partial sequence similarity to CD11d and may cross react.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD11b

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD11b antibody (ABIN730918)

Yamaguchi, Sato, Kato-Itoh, Goto, Hara, Sanbo, Mizuno, Kobayashi, Yanagida, Umino, Ota, Hamanaka, Masaki, Rashid, Hirabayashi, Nakauchi: "Interspecies organogenesis generates autologous functional islets." in: Nature, Vol. 542, Issue 7640, pp. 191-196, (2017) (PubMed).

Zhang, Diao, Jia, Yuan, Thamm, Wang, Jin, Pei, Zhou, Yu, Zhao, Cheng, Du, Huang, Zhang, Lin: "Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model." in: PLoS ONE, Vol. 12, Issue 12, pp. e0188960, (2017) (PubMed).

Long, Luo, Wang, Bates, Shetty: "Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells." in: Aging and disease, Vol. 8, Issue 3, pp. 301-313, (2017) (PubMed).

Velázquez, Enos, McClellan, Cranford, Chatzistamou, Singh, Nagarkatti, Nagarkatti, Fan, Murphy: "MicroRNA-155 deletion promotes tumorigenesis in the azoxymethane-dextran sulfate sodium model of colon cancer." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 6, pp. G347-58, (2016) (PubMed).

Kobayashi, Tanizaki, Kimura, Ishida, Nosaka, Toujima, Kuninaka, Minami, Ino, Kondo: "AG490, a Jak2 inhibitor, suppressed the progression of murine ovarian cancer." in: European journal of pharmacology, Vol. 766, pp. 63-75, (2015) (PubMed).

Zhang, Hu, Liu, Ye, Gui, Zhou, Qi, He, Wang, Wang: "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." in: Scientific reports, Vol. 5, pp. 15948, (2015) (PubMed).

Liu, Xie, Ma, Zhang, Liang, Shi, Yu, Feng, Zhang, Wu: "Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation." in: Scientific reports, Vol. 4, pp. 4833, (2014) (PubMed).

Yu, Yang, Wang, Chen, Lu, Luo, Wang: "Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma." in: Alcoholism, clinical and experimental research, Vol. 38, Issue 10, pp. 2597-606, (2014) (PubMed).

Long, Qiu, Wang, Yang, Jia, Xin, Wang, Han, Fei, Liu: "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." in: Brain research, Vol. 1532, pp. 1-13, (2013) (PubMed).

Long, Qiu, Liu, Fei, Feng, Wang, Zhong, Yi, Liu, Zhang, Han: "Functional recovery and neuronal regeneration of a rat model of epilepsy by transplantation of Hes1-down regulated bone marrow stromal cells." in: Neuroscience, Vol. 212, pp. 214-24, (2012) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b, CD11c

Background:

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, ITGAM, Integrin alpha-X, ITGAX, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD11c

Background: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation