Midkine antibody

Catalog No. ABIN7642853

The Rabbit Polyclonal anti-Midkine antibody is suitable to detect Midkine in samples from Rat. It has been validated for WB.

Quick Overview for Midkine antibody (ABIN7642853)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Midkine Antibody

Purpose: Polyclonal Antibody to Midkine (MK)

Specificity: The antibody is a rabbit polyclonal antibody raised against MK. It has been selected for its ability to recognize MK in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA631Ra01Recombinant Midkine (MK)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500
Immunohistochemistry: 5-20 μg/mL,1:25-100
Immunocytochemistry: 5-20 μg/mL,1:25-100
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: Midkine

Background: MDK, ARAP, NEGF2, Neurite Growth-Promoting Factor 2, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2

UniProt: Q9R1S9

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development