ASK1 antibody

Catalog No. ABIN7642910

The Rabbit Polyclonal anti-ASK1 antibody is suitable to detect ASK1 in samples from Mouse. It has been validated for WB and IHC.

Quick Overview for ASK1 antibody (ABIN7642910)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ASK1 Antibody

Purpose: Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ASK1. It has been selected for its ability to recognize ASK1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB358Mu01Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: MAP3-K5, MAP3K5, ASK1, MAPKKK5, MEKK5, Mitogen-Activated Protein Kinase Kinase Kinase 5, MAPK/ERK kinase kinase 5

UniProt: O35099

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response