NONO antibody

Catalog No. ABIN7643652

This anti-NONO antibody is a Rabbit Polyclonal antibody detecting NONO in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for NONO antibody (ABIN7643652)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NONO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NONO Antibody

Purpose: Polyclonal Antibody to Non POU Domain Containing Octamer Binding Protein (NONO)

Specificity: The antibody is a rabbit polyclonal antibody raised against NONO. It has been selected for its ability to recognize NONO in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPJ818Hu01Recombinant Non POU Domain Containing Octamer Binding Protein (NONO)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: NONO

Background: P54, NMT55, NRB54, P54NRB, Nuclear RNA-Binding Protein 54-kD, 54 kDa nuclear RNA- and DNA-binding protein, DNA-binding p52/p100 complex, 52 kDa subunit

UniProt: Q15233