NF-kB p65 antibody

Catalog No. ABIN7643693

The Mouse Monoclonal anti-NF-kB p65 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect NF-kB p65 in samples from Rat.

Quick Overview for NF-kB p65 antibody (ABIN7643693)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NF-kB p65 Antibody

Purpose: Monoclonal Antibody to Transcription Factor P65 (NFKB3)

Specificity: The antibody is a mouse monoclonal antibody raised against NFKB3. It has been selected for its ability to recognize NFKB3 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPA616Ra01Recombinant Transcription Factor P65 (NFKB3)

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NFKB3

Background: Nuclear factor NF-kappa-B p65 subunit, NFKBP65, RELA, p65, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins