PCK1 antibody

Catalog No. ABIN7644272

The Rabbit Polyclonal anti-PCK1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PCK1 in samples from Mouse.

Quick Overview for PCK1 antibody (ABIN7644272)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PCK1 Antibody

Purpose: Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PCK1. It has been selected for its ability to recognize PCK1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background: PEPCK1, PEPCKC, Cytosolic Phosphoenolpyruvate Carboxykinase, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

UniProt: Q9Z2V4

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis