PFKM antibody

Catalog No. ABIN7644280

This Mouse Monoclonal antibody specifically detects PFKM in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for PFKM antibody (ABIN7644280)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PFKM Antibody

Purpose: Monoclonal Antibody to Phosphofructokinase, Muscle (PFKM)

Specificity: The antibody is a mouse monoclonal antibody raised against PFKM. It has been selected for its ability to recognize PFKM in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM

Background: PFK1, PFKX, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, 6-Phosphofructokinase Polypeptide X, Fructose-6-Phosphate 1-Phosphotransferase

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect