RHOA antibody

Catalog No. ABIN7645290

The Rabbit Polyclonal anti-RHOA antibody (ABIN7645290) specifically detects RHOA in WB, IHC, ICC and IP. The antibody is reactive with Human samples.

Quick Overview for RHOA antibody (ABIN7645290)

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-RHOA Antibody

Purpose: Polyclonal Antibody to Ras Homolog Gene Family, Member A (RHOA)

Specificity: The antibody is a rabbit polyclonal antibody raised against RHOA. It has been selected for its ability to recognize RHOA in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPE320Hu01Recombinant Ras Homolog Gene Family, Member A (RHOA)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RHOA

Background: ARH12, ARHA, RHO12, RHOH12, Rho cDNA clone 12

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome