SPI1 antibody

Catalog No. ABIN7646564

The Rabbit Polyclonal anti-SPI1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SPI1 in samples from Rat.

Quick Overview for SPI1 antibody (ABIN7646564)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SPI1 Antibody

Purpose: Polyclonal Antibody to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SPI1. It has been selected for its ability to recognize SPI1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1

Background: OF, PU.1, SFPI1, SPI-1, SPI-A, SPI-1 Proto-Oncogene, Hematopoietic Transcription Factor PU.1, 31 kDa Transforming Protein

UniProt: Q6BDS1

Pathways: Stem Cell Maintenance